Fluorimetric method for measuring chemotaxis.

found that all the antibodies mentioned above showed equally good staining results on our smears. In normal adults the number of peripheral blood lymphocytes stained by these markers were as follows: Tll: 75-90%; Bi: 10-20%; 12: 10-20%; T4: 55-65%; T8: 25-35%; J5: 100% negative. These figures are in close agreement with those given by the manufacturer when using immunofluorescence. Using this method we have successfully demonstrated various surface markers on normal lymphocytes and leukaemic cells in acute lymphoblastic leukaemia, lymphoma, and chronic lymphocytic leukaemia. We are aware that the immunofluorescence method is equally good for demonstrating cellular antigens on smeared cells, and a report describing such technique has recently been published in the Journal ofClinical Pathology.7 The advantage of the method described in the present paper over immunofluoresence is that a simultaneous study of morphology and immunocytochemistry is possible and the stained smears can be filed as a permanent record. 709